Title:
Evaluation of the Effect of Recombinant GnRH Vaccine on the Reproductive Function Through Specific Antibody, Hormones, Testis Structure, Sperm Quality, and Fertilization in Adult ICR Male Mice
Evaluation of the Effect of Recombinant GnRH Vaccine on the Reproductive Function Through Specific Antibody, Hormones, Testis Structure, Sperm Quality, and Fertilization in Adult ICR Male Mice
Time:
06/19 (Sat.) 8 pm PDT, 9 pm MDT, 10 pm CDT, 11 pm EDT
06/20 (Sun.) 5 am CEST, 11 am Taiwan
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06/19 (Sat.) 8 pm PDT, 9 pm MDT, 10 pm CDT, 11 pm EDT
06/20 (Sun.) 5 am CEST, 11 am Taiwan
Time zone conversion tool
Keywords:
fertility control, Immunocontraception
fertility control, Immunocontraception
Abstract:
The secretion of gonadotropin-releasing hormone (GnRH) regulates the reproductive endocrine system. The GnRH immunocontraception vaccine has been recognized as a potential tool for animal population control and management. We aimed to evaluate a recombinant GnRH fusion protein with MONTANIDE™ ISA 206 VG adjuvant as GnRH vaccine in adult ICR male mice based on anti-GnRH antibodies, concentrations of the sexual hormone, size and histomorphology of testis, semen quality. After intramuscular administration of vaccine to mice in weeks 0, 4, and 8, the response was assessed and observed that the inducing of the specific immune response at week 5 and maintain the specific antibody for at least weeks 24. Furthermore, our vaccine suc-cussed reducing the concentration of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone in serum. In addition, we observed that the atrophy of testis and decreased quality of sperm in concentration, sperm morphology, and viability in the vaccine group. Through the fertility test, we found that this vaccine decreases the mating and pregnancy rate. The vaccine in this study could be used as a tool for population management of free-living animals. However, future studies that evaluation the effect of the vaccine in specific species and in the field are needed.
The secretion of gonadotropin-releasing hormone (GnRH) regulates the reproductive endocrine system. The GnRH immunocontraception vaccine has been recognized as a potential tool for animal population control and management. We aimed to evaluate a recombinant GnRH fusion protein with MONTANIDE™ ISA 206 VG adjuvant as GnRH vaccine in adult ICR male mice based on anti-GnRH antibodies, concentrations of the sexual hormone, size and histomorphology of testis, semen quality. After intramuscular administration of vaccine to mice in weeks 0, 4, and 8, the response was assessed and observed that the inducing of the specific immune response at week 5 and maintain the specific antibody for at least weeks 24. Furthermore, our vaccine suc-cussed reducing the concentration of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone in serum. In addition, we observed that the atrophy of testis and decreased quality of sperm in concentration, sperm morphology, and viability in the vaccine group. Through the fertility test, we found that this vaccine decreases the mating and pregnancy rate. The vaccine in this study could be used as a tool for population management of free-living animals. However, future studies that evaluation the effect of the vaccine in specific species and in the field are needed.
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